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Ligands Lectins Concanavalin A Heparin Ni-NTA Calmodulin ssDNA Triazine dyes: Cibacron blue 5 -AMP Protein A MAB Glutathione Amylose Target molecules α-D-glucopyranosyl α-D-mannopyranosyl Nucleic acid binding proteins Growth factors His6-tag Calmodulin- dependent enzymes DNA-dependent enzymes NAD, NADP- dependent enzymes ATP-dependent enzymes Immunoglobulins Antigens Glutathione S transferases Maltose binding protein Examples Adenosine deaminase γ-Glutamyl transferase DNA helicases Restriction endonucleases DNARNA polymerase Endothelial cell GF N- or C-terminus (His)6 ATPase DNA-polymerases alkyl transferase Dehydrogenases Quinone reductases cAMP-dependent protein kinase Fc region of IgGs Protein A fusions Glutathione S transferase fusions MBP fusions CLASSICALCLONING,EXPRESSION,ANDPURIFICATION 21 Modified from Skelly and Madden (1996).
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